Microbeads
TCRg/d+ T Cell Isolation Kit, mouse
The TCRγ/δ+ T Cell Isolation Kit has been developed for the isolation of mouse TCRγ/δ+ T cells from single-cell suspensions of spleen, lymph nodes, or thymus.
Quantity
T cells bearing γ/δ TCRs are a phenotypically and functionally conserved lineage of T cells which often constitutes the major T cell population in epithelial-rich tissues, e.g. intestine, skin, lung, and reproductive tract. In blood and lymphoid tissues they are found at low frequency. TCRγ/δ
+
T cells have been shown to be involved in immune surveillance against tumors, wound healing, as well as pro- and anti-inflammatory responses against pathogens.
1The TCRγ/δ
+
T Cell Isolation Kit contains CD11b and CD45R (B220) MicroBeads for depletion of CD11b
+
and CD45R (B220)
+
cells, as well as Anti-TCRγ/δ-Biotin and Anti-Biotin MicroBeads for positive selection of TCRγ/δ
+
T cells.
Downstream applications
TCRγ/δ
+
T cells isolated by using the TCRγ/δ
+
T Cell Isolation Kit are suitable for phenotypic and functional analysis, adoptive transfer, and coculture experiments.
Columns
For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.