Detailed separation procedure
Non-plasma cells are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies and Anti-Biotin MicroBeads. The labeled cells are subsequently depleted by separation over a MACS Column. In the second step, pre-enriched plasma cells (flow-through fraction) are directly labeled with CD38 MicroBeads and isolated by positive selection from the pre-enriched plasma cell fraction.