Microbeads
Anti-FITC MicroBeads
Anti-FITC MicroBeads are widely used for indirect magnetic labeling and separation of cells or other materials which are labeled with a primary antibody or ligand conjugated to fluorescein isothiocyanate (FITC).
Quantity
The FITC molecule is recognized by the monoclonal anti-FITC antibody coupled to MicroBeads, allowing the positive selection or depletion of the fluorescently labeled material with MACS
®
Technology. Labeling samples with FITC-conjugated primary antibodies and Anti-FITC MicroBeads enables subsequent flow cytometric analysis of separated cells without any further staining of the material.
Applications
Examples among the many separations performed with Anti-FITC MicroBeads are the isolation of IgD
+
B cells from human PBMCs
, the separation of plant protoplasts
as well as the isolation of murine Langerhans cells from epidermal tissue
. Anti-FITC MicroBeads have also been used for the depletion of CD3
+
cells from rat spleen and subsequent isolation of CD3
-
NK cells
or the separation of murine naive CD8
+
T cells expressing low levels of CD44 by depletion of CD44
int/high
cells
.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.