Microbeads
CD11b MicroBeads, human and mouse - lyophilized
CD11b MicroBeads were developed for the isolation or depletion of human and mouse cells based on their CD11b expression.
Quantity
Background information
In humans, CD11b is strongly expressed on myeloid cells, and weakly expressed on NK cells and some activated lymphocytes.
In mice, the CD11b antigen is expressed on monocytes/macrophages and to a lower extent on granulocytes, NK cells, CD5
+
B1 cells, and a subset of dendritic cells.
Downstream applications
In humans, CD11b MicroBeads can be used for positive selection or depletion of CD11b
+
cells from PBMCs, bone marrow, and other lymphoid tissues. They are mostly used in combination with other MicroBeads for depletion of unwanted cells.
In mice, CD11b MicroBeads have been used to isolate CD11b
+
cells from cell suspensions of spleen
1
, lymph node
2
, peritoneal cavity
3
, liver
4
, muscle tissue
2
, or blood
5
. They were also used in combination with other MicroBeads for the enrichment of mast cells from peritoneal cell suspensions.
3For the isolation of human monocytes CD14 MicroBeads or the Pan Monocyte Isolation Kit are recommended.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.