Microbeads
CD22 MicroBeads, human
CD22 MicroBeads were developed for positive selection or depletion of human B cells from PBMCs, bone marrow, or single-cell suspensions from lymphoid tissues.
Quantity
In peripheral blood, cell surface expression of the CD22 antigen is found on mature B cells, but is lost during terminal differentiation to plasma cells. In bone marrow, surface expression of CD22 can be detected from the pre-B cell stage on. Cytoplasmic expression of CD22 can also be found in late pro-B cells and early pre-B cells.
Downstream applications
The purified B cells may be used for cell biological studies, such as analysis of signal transduction
, mixed lymphocyte responses
, antigen presentation, or for molecular studies, e.g. PCR analysis
.
Columns
For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.