Microbeads
CD8a MicroBeads, rat
Rat CD8a MicroBeads have been developed for positive selection or depletion of cytotoxic T cells from lymphoid organs, blood, or other tissues.
Quantity
Overview
Rat CD8a MicroBeads have been developed for positive selection or depletion of cytotoxic T cells from lymphoid organs, blood, or other tissues.
Detailed product information
Background information
CD8a MicroBeads recognize the immunoglobulin-like domain of the CD8α chain, which is expressed as a heterodimer with CD8β on TCRα/β
+
and TCRγ/δ
+
cytotoxic T cells and on most thymocytes.
As a homodimer of two CD8α chains, the CD8 antigen is expressed on most NK cells,
a major fraction of intestinal intraepithelial lymphocytes, on some activated CD4
+
CD8
+
double-positive T cells, and on CD8
+
T cells from athymic rats. CD8a is also expressed by a T cell subset with regulatory functions.
Downstream applications
Thymocytes or mature T cells isolated with CD8a MicroBeads can be used for
in vitro
analysis
or
in vivo
reconstitution
of T cells, MHC restriction, tolerance induction, and cytokine expression experiments. Functionality of the isolated cells has been shown by several adoptive transfer experiments. Transfer of isolated CD4
+
and CD8a
+
T cells into irradiated recipients results in donor-specific acceptance to heart transplants
or type V collagen-induced tolerance to lung allografts
. Furthermore, autoimmunity and induction of tolerance after xenogeneic thymus transplantation was studied by adoptive transfer of T cells separated by MACS
®
Technology.
Columns
For positive selection: MS, LS, XS, or autoMACS
® Columns. For depletion: LD, D, or autoMACS Columns.