Background information
CD25, the low-affinity interleukin-2 receptor alpha chain (IL-2Rα), is expressed on activated T and B cells and monocytes. CD25 is also present on a subset of non-activated CD4
+
T cells, which has regulatory function.
The CD25 MicroBeads II recognize epitope A of the CD25 molecule.
For control staining of cells separated with CD25 MicroBeads II, it is recommended to use MACS
®
CD25 Antibodies, which bind to epitope B of the CD25 antigen.
For isolation of CD25
+
cell subsets, the CD25 MicroBeads II can be combined with MACS
®
Cell Isolation Kits or MultiSort Kits. The CD4
+
CD25
+
Regulatory T Cell Isolation Kit is recommended for most efficient separation of CD4
+
CD25
+
regulatory T cells.